Key: Click on "details" for more information on a particular experiment or article. "Result" is a short description of the experiment result. "Methods" use a ontology to describe the methods of the experiment.
  Results:Regulatory site mappingMethods:Gel retardation
In order to determine if FruR directly binds to the ptsHI-crr operon, a 484 bp DNA fragment encompassing the regulatory region of this operon was used in a DNA band migration retardation assay. This fragment was retarded when FruR was present. The addition of fructose-1-phosphate to the binding reaction prevented the binding of FruR to the pts regulatory region.
Experiment Elements
site: FruR retarded region at pts operon Escherichia coli
regulator: FruR
operon: pts Escherichia coli
effector: Fructose-1-phosphate
Ramseier TM,N+ègre D,Cortay JC,Scarabel M,Cozzone AJ,Saier MH
In vitro binding of the pleiotropic transcriptional regulatory protein, FruR, to the fru, pps, ace, pts and icd operons of Escherichia coli and Salmonella typhimurium.J Mol Biol 1993 Nov;234(1):28-44. (details)
PubMed: 8230205