Experiments

Key: Click on "details" for more information on a particular experiment or article. "Result" is a short description of the experiment result. "Methods" use a ontology to describe the methods of the experiment.
  Results:Regulatory site mappingMethods:Footprinting
DNase I protection experiments were conducted to establish the precise location of cAMP-CRP binding to the regulatory region of the ogl gene. A single protected region was obtained. This region contained cAMP-CRP binding site centred at position -98.5 with respect to the ogl transcription start site (P1) and -41.5 for ogl P2. For the ogl promoter, the KdgR-protected region was obtained to overlap the cAMP-CRP binding site.
Experiment Elements
site: KdgR protected fragment at ogl gene Dickeya dadantii 3937
site: CRP protected region at ogl gene Dickeya dadantii 3937
gene: ogl Dickeya dadantii 3937
regulator: KdgR Dickeya dadantii 3937
regulator: CRP Dickeya dadantii 3937
Article
Nasser W,Robert-Baudouy J,Reverchon S
Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes.Mol Microbiol 1997 Dec;26(5):1071-82. (details)
PubMed: 9426143