Key: Click on "details" for more information on a particular experiment or article. "Result" is a short description of the experiment result. "Methods" use a ontology to describe the methods of the experiment.
  Results:Regulatory site mappingMethods:Footprinting; Mutagenesis
Occupancy of half-site on fragment with mutant CtrA-binding d site and on wild-type Cori fragment was quantified in DNase I footprinting experiments. At site d on wild-type Cori fragment CtrA simultaneously bound both left and right half-sites with Kd 0.25 mkM. At site d on mutant fragment CtrA bound only the right half-site but with lower affinity and Kd 0.6 mkM.
Experiment Elements
site: CtrA-binding site d at Cori Caulobacter vibrioides
site: mutant Cori CtrA-binding site d artificial sequences
regulator: CtrA Caulobacter vibrioides
Siam R,Marczynski GT
Cell cycle regulator phosphorylation stimulates two distinct modes of binding at a chromosome replication origin.EMBO J 2000 Mar;19(5):1138-47. (details)
PubMed: 10698954